Tg(Prnp-MAPT*P301S)50Hiw

The mouse prion promoter drives the expression of the longest human tau (MAPT) isoform (4R2N) in which proline is replaced by a serine at amino acid position 301 of the protein (P301S). Seven lines were established (16, 50, 56, 72, 82, 96, and 100). Line 50 is the highest expressing line. Western blot analysis confirmed increased expression in the hippocampus and showed that human tau is phosphorylated at T181, S202/T205, S214, S262, and S396. (J:213361)