这个等位基因是通过shotgun CRISPR/Cas9编辑通过设计针对Pcdhg基因簇每个可变外显子的pool sgRNAs产生的,且这个特定的等位基因在可变外显子B8仅发现了一个框移的插入突变。 (Source: The allele was generated through CRISPR/Cas9 with pooled sgRNAs targeting Pcdhg variable exons, and it had a frameshifting indel in exon B8.)
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This allele was generated through shotgun CRISPR/Cas9 editing using pooled sgRNAs that targeted each variable exon of the Pcdhg cluster and this allele was found to have a frame shifting indel only in variable exon B8.