这个等位基因是通过shotgun CRISPR/Cas9编辑通过设计针对Pcdhg基因簇每个可变exon的pool sgRNAs产生的,检测发现它存在帧移或其它变异,这些变异影响了可变exons A3、A10和C4。 (Source: The allele was generated through CRISPR/Cas9 with pooled sgRNAs targeting Pcdhg's variable exons, and it was found to have frame shifts or mutations disrupting exons A3, A10, and C4.)
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This allele was generated through shotgun CRISPR/Cas9 editing using pooled sgRNAs that targeted each variable exon of the Pcdhg cluster and this allele was found to have frameshift or other mutations disrupting the variable exons A3 and A10 and C4.