Kirrel2^{tm2b(KOMP)Wtsi}

The L1L2_Bact_P cassette was inserted at position 30154397 of Chromosome 7 upstream of the critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 30155529. Exons 3 and 4 are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm2a allele to remove the neo selection cassette and loxP-flanked exons 3 and 4. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. In situ hybridization and immunohistochemical analyses confirmed the absence of transcript and protein expression in the vomeronasal organ (VNO) and on vomeronasal sensory neuron (VSN) axons of homozygous mutant mice, respectively. (J:272414)