使用了一种从5'到3'含有loxP-F3-puromycin Δtk-loxP-FRT靶向序列的载体,用于在染色体5上敲除Mir574。Mir574位于宿主基因Fam114a1的内含子1位置。通过Cre介导的重组,去除了含有的floxed puromycin元件。(来源:J:174268)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count