Reck^em2Nat

CRISPR/Cas9 genome editing is used to insert alanine substitution mutations P256A (nucleotide change: CCT to GCA) and W261A (nucleotide change: TGG to GCC). The mutations eliminate Wnt7a signaling by the Reck gene, but do not affect protein folding or trafficking to the plasma membrane. (J:277288)