CRISPR/Cas9技术被用来注射了4个gRNA,其中两个针对exon 1,另两个针对exon 2,目标序列区域被删除。 Western blot结果显示没有检测到蛋白质。(来源:J:271250)
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CRISPR/Cas9 technology was used to inject 4 gRNAs, two gRNAs targeting exon 1 and the other two to exon 2. Sequence between the gRNA targeting sites was deleted. Western blot analysis confirmed absence of protein. (J:271250)