Fgl1^{tm1b(EUCOMM)Hmgu}

The L1L2_Pgk_P cassette was inserted at position 41662443 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, an SV40 polyA, a second FRT site, and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 41663288. The critical exon(s) is/are thus flanked by loxP sites. Cre mediated recombination without flp expression created a reporter knockout mouse. ELISA and western blot analyses confirmed the absence of protein in the plasma and liver, respectively, from homozygous mice. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:269578)