Smcr8^em2Btlr

The CRISPR/Cas9 system and sgRNA 5'-GATCAGCGCCCCTGATGTGG-3' was used. Mice contain three separate point mutations on chromosome 11: 60,778,032 (T>C), 60,778,048 (G>A), and 60,778,078 (G>A). The first mutation results in an isoleucine-to-threonine change in amino acid 2 (p.I2T) of the SMCR8 protein while the latter two are silent and result in no coding changes. (J:268243)