构建了一个替代载体(TRE-tGFP-miRE\Sod2_835_shRNA_1),包含FRT位点、 Tet响应元件、人类CMV的constitutive活跃、最小的启动子,以及IRES-turboGFP序列(快速成熟、短半衰期的绿色荧光蛋白变体);Sod2_835_shRNA_1被置于tGFP的3' UTR,跟着RBGpolyA序列和另一个FRT位点。Sod2_835_shRNA_1包含一个141bp的shRNA序列,能显著在mRNA和蛋白水平上减少SOD2的表达。载体插入Col1a1基因座。(来源:J:267914)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x 129S4/SvJae)F1
Targeted
插入
--
1
24
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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