在第1和2个内含子中插入了LoxP位点。随后的Cre介导重组删除了exon 2,产生了敲除等位基因。 Western blot结果显示该位点处没有检测到蛋白质表达。(来源:J:267563)
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LoxP sites were inserted into introns 1 and 2. Subsequent cre-mediated recombination deleted exon 2, creating a knockout allele. Western blots confirmed the absence of protein expression from the locus. (J:267563)