通过同源重组,用PGK-诺美霉素插件替换了2-4号外显子。RT-PCR证实了肝脏和肾脏中没有mRNA, Western blot分析也证实了肝脏和肾脏中没有相应蛋白(来源:J:267178)。
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A PGK-Neomycin cassette replaced exons 2-4 via homologous recombination. RT-PCR confirmed absence of mRNA in liver and kidney and Western blot analysis confirmed absence of protein in liver and kidney. (J:267178)