A loxP site was inserted upstream of the exon 4, and an frt flanked neo cassette and another loxP site was inserted downstream of the exon 4. Flp mediated recombination removed the neo cassette leaving the coding exon floxed. (J:232100)
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A loxP site was inserted upstream of the exon 4, and an frt flanked neo cassette and another loxP site was inserted downstream of the exon 4. Flp mediated recombination removed the neo cassette leaving the coding exon floxed. (J:232100)