Del(3Bglap2-Bglap)1Vari

CRISPR/Cas9 technology is used to delete exon 2 of Bglap through exon 4 of Bglap2 (6.8 kb). This deletion leaves the first 4 amino acids of exon 2 of Bglap intact followed by a 5bp insertion and a portion of exon 4 of Bglap2. The mutation is predicted to create a reading frame shift after the 25th amino acid and a termination codon 17 residues further downstream. Mature protein is undetectable in homozygous animals. (J:289623)