将lacZ/Neo cassette替换了2号和3号外显子。这个插入预测会导致在交换器域之前发生早早期截断,或者通过非编码介导的mRNA降解。 Western blot分析证实了蛋白质丢失。(来源:J:262453)
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A lacZ/Neo cassette replaced exons 2 and 3. This insertion is predicted to cause an early truncation before the exchanger domain and/or non-sense-mediated mRNA decay. Western blot analysis confirmed loss of protein. (J:262453)