Crb1^rd8+em1Boc

TALEN targeting of exon 9 for homologous recombination, using the same single stranded oligonucleotides used to generate the rd8+em1Mvw revertant allele but mediated in fertilized oocytes from a different strain, successfully replace the deleted nucleotide (C/G) in the retinal degeneration 8 (rd8) mutation and incorporates 5 synonymous base substitutions inside the TALEN binding regions. (J:265859)