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The L1L2_Bact_P cassette was inserted upstream of the targeted exon. The cassette is composed of an FRT site followed by a splice acceptor site, IRES, a lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, an SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon. The targeted exon is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:265802)