CRISPR/cas9介导的重组在exon 2产生了一个19bp的缺失。 Western blot分析显示,纯合小鼠脑匀浆中没有蛋白质表达。(来源:J:265620)
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CRISPR/cas9 mediated recombination created a 19bp deletion in exon 2. Western blot analysis indicated the complete absence of protein expression in brain homogenates from homozygous mice. (J:265620)