Gt(ROSA)26Sor^{tm1(CAG-EGFP)Maito}

A construct containing the CAG promoter with a VloxP-STOP-VloxP cassette preceding EGFP sequence was inserted into the intron of the Gt(ROSA)26Sor locus via homologous recombination in Wit 9 ES cells. VCre-mediated recombination of this allele results in the expression of the EGFP reporter driven by the CAG promoter. VCre/VloxP is a site-specific recombination system, which was derived from plasmid p0908 of Vibrio sp. VCre is a recombinase that recognizes its own specific target sites (VloxP). (J:263660)