Exon 3周围标记了loxP位点,接着是带有IRES-tandem-dimer红色荧光蛋白和polyA报告基因的构建,同时3'UTR插入了FRT引导的neo cassette,通过同源重组实现。随后,Flp介导的重组去除了neo cassette。(来源:J:265419)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count