Gt(ROSA)26Sor^{tm1(CAG-Syp/EYFP)Htng}

The targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), an FRT-flanked STOP cassette (with a short open reading frame, followed by translational stops in all three reading frames, a synthetic polyA sequence, a HGH polyA sequence and a HSV-TK polyA sequence), a loxP-flanked STOP cassette (with stop codons in all three reading frames linked to three repeats of the SV40 polyA signal), the SypYFP fusion gene (a mouse synaptophysin [Syp] cDNA fused to an enhanced yellow fluorescent protein sequence), a woodchuck hepatitis virus post-transcriptional regulatory element, a BGH polyA signal, an AttB site, a PGK promoter-Neomycin resistance gene-PGK pA cassette and an AttP site. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:101977)