Hcrt^{tm1.1(cre)Ldl}

Bacterial artificial chromosome clones containing the preprohypocretin (Hcrt) gene were used to target a cassette containing the internal ribosomal entry sequence (IRES) linked to Cre recombinase and a Frt-flanked neomycin resistance gene immediately downstream of the exon 2 stop codon. The target construct was electroporated into C57BL/6 embryonic stem cells, targeted clones were injected into blastocysts, and resultantchimeras were bred for germline transmission and then crossed with Flp-deletion mice to remove the neomycin selection gene. (J:264534)