Tg(Ntrk2-cre/ERT2)#Phep

A cassette containing a modified CreERT2-pA-Frt-Ampicillin-Frt was inserted in the first coding ATG of exon 2 into a bacterial artificial chromosome (BAC) containing the Ntrk2 mouse locus (RP23-391J8) deleting the entire coding region of exon 2. Purified BAC cDNA was purified and injected into pronuclei of B6D2F1/N oocytes or prepared for intracytoplasmic sperm injection (ICSI). Both methods produced transgenic offspring. Line numbers were not indicated. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:265202)