在exon 5的止吐密码子(STOP)之前,插入了RAKR的furinmotif序列来修剪残留的2A肽,这个序列是GCG(甘氨酸-丝氨酸-甘氨酸)间隔,FMDV 2A肽(F2A)的序列,EGFP以及FRT引导的诺如霉素抗性(neo)基因。随后,通过Flp介导的重组,去除了选择性 cassette。(来源:J:239547)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
插入
--
1
2
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
Show/Hide columns
Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
微信
信息比对
科研助手
使用教程
回到顶部