在exon 5的止吐密码子(STOP)之前,插入了RAKR的furinmotif序列来修剪残留的2A肽,这个序列是GCG(甘氨酸-丝氨酸-甘氨酸)间隔,FMDV 2A肽(F2A)的序列,EGFP以及FRT引导的诺如霉素抗性(neo)基因。随后,通过Flp介导的重组,去除了选择性 cassette。(来源:J:239547)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count