Dync1h1^tm1.1Sjki

Exon 5 was replaced with one containing an A to G point mutation resulting in a histidine to arginine change at amino acid 304 (H304R) that corresponds to the human H306R mutation seen in Charcot-Marie-Tooth type 2O patients. A FRT-flanked neomycin resistance cassette was inserted in intron 5-6 and was removed via Flp-mediated recombination. Western blot analysis confirmed expression of the protein. (J:264493)