Gnptab^tm1.2Ddray

A targeting vector was designed to replace exon 19 with one specifying a change of glutamic acid to lysine at amino acid position 1179 (c.3535G>A (GAA-AAA)) , which is homologous to a human Glu1200Lys mutation correlated with stuttering. The targeting vector also contained an internal ribosome entry site (IRES) to allow initiation of translation of an inserted beta-galactoside gene (beta-gal) for subsequent gene expression studies in tissues, as well as a neomycin resistance cassette (PGK-Neo-pA) for selection of the transfected ES cells. Crossing mice with the targeted allele to a flp-expressing line removed the neomycin resistance cassette, and subsequent crossing into a cre-expressing line removed the IRES- gal component to generate BALB/c animals carrying the mutant exon 19 containing Glu1179Lys. (J:260041)