Abcc6^{tm1c(EUCOMM)Wtsi}

The L1L2_Bact_P cassette was inserted at position 46019130 of Chromosome 7 upstream of exon 5. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 46017355. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exons 5 and 6 floxed. (J:252837)