A 67 bp deletion was created using sgRNAs 5'-GTACGCCGTGCGCACCCTCT-3' and 5'-GGCGCGGCCGGCACAGATAG-3' and CRISPR/Cas9 technology. This deletion in the second exon crates a frameshift and premature stop codon. As a result virtually the entire homeodomain is eliminated. (J:252916)