Hoxd10^em1Ddu

Starting with genomic DNA from the Del(2Hoxd1-Hoxd9)63Ddu allele, a 10 bp deletion (CCGACAGGCA) was created Using sgRNA 5'-AGAGCGTTAACCTCACCGAC,-3'. This deletion affects the coding region, introducing a frameshift and premature stop codon that replaces the last 34 amino-acids of the encoded peptide with 10 new amino-acids. This truncated peptide has a disrupted homeodomain with the third alpha-helix eliminated. (J:252916)