Nfia^tm1.1Elgaz

The EUCOMM PGRS0001-B-F10 targeting vector containing a FRT site-flanked splice-acceptor- LacZ-loxP-Neo cassette, and loxP sites flanking exon 2, was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 2. Flp-mediated recombination removed the FRT-flanked LacZ/neo cassette leaving exon 2 floxed. (J:244204)