Spata25^{tm1b(EUCOMM)Hmgu}

The Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 164669246 of Chromosome 2 (Build GRCm39) within an endogenous exon, creating an artificial intron that splits the original exon into two artificial exons, where the downstream artificial exon is critical. The cassette is composed of the intron of the Ifitm2 gene containing an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 164669851. The critical artificial exon(s) is/are thus flanked by loxP sites. Cre mediated recombination without flp expression produced a reporter knockout mouse. Further information on targeting strategies used for this and other EUCOMMTOOLs alleles can be found at http://www.knockoutmouse.org/about/eucommtools/vectors. (J:234235)