Sequences include CMV enhancer/chicken beta-actin promoter (CAG), a loxP-STOP(3xSV40 polyA)-loxP cassette (LSL), full-length mouse Arl13b cDNA (OTTMUST00000058920.GRCm38) fused to mCerulean and Fucci2a using the porcine teschovirus-1 2A (P2A) ribosomal skip cleavage peptide sequence on the N-terminus of the Fucci2a cell cycle probe consisting of a fusion of mCherry with a truncated human CDT1 (aa 30-120) fused to mCherry and mVenus fused to 110aa N-terminal human Geminin fragmentand a bovine growth hormone polyA sequence (BGHpA). Cre-mediated recombination removed the STOP cassette. (J:267034)