将靶向构建插入到第3外显子,包含taulacZ报告基因融合和敲除neo抗性基因座的IRES序列。 Western blot结果显示大脑中未检测到蛋白表达。(来源:J:263658)
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The targeting construct introduced into exon 3 an IRES sequence, taulacZ reporter fusion gene and floxed neomycin resistance cassette. Western blot analysis confirmed the absence of protein expression in the brain. (J:263658)