Exon 2 was replaced with an attB53 site, human sequenced (fusion of exon 2 through 4, intronic and exonic sequence from intron 4 through 7, and a fusion of exons 8 and 9), TGA stop codon, FRT-flanked hygromycin resistance cassette, and attP50 site. Flp-mediated recombination removed the selection cassette. (J:263525)