CRISPR/Cas9介导的重组产生了44个碱基对的缺失(等位基因c.58-102del),位于exon 2。 Western blot结果显示,纯合小鼠睾丸中没有检测到蛋白表达。(来源:J:263089)
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CRISPR/Cas9 mediated recombination produced a 44 bp deletion (c.58-102del) in exon 2. Western blot analysis confirmed the absence of protein expression in testes from homozygous mice. (J:263089)