在第一个编码外显子中插入了GFP-Cre/ERT2基因驱动元件和FRT引导的kanamycin/neomycin选择标记 cassette,通过RT-PCR和 Western blot 验证了纯合子中没有mRNA和蛋白质表达。(来源:J:251841)
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A GFP-Cre/ERT2 cassette and FRT-flanked Kanamycin/neomycin selection cassette were inserted into the first coding exon. RT-PCR and Western blot analysis confirmed absence of mRNA and protein, respectively, in homozygotes. (J:251841)