The transposon containing an unspecified hybrid actin promoter driving expression of red fluorescent protein (RFP) flanked by piggyBac-specific left and right terminal sequences was co-injected with a piggyBac-containing transgene to mobilize the transposon. This line contains a transposon inserted into intron 14. Immunohistochemical analysis confirmed the absence of protein in olfactory cilia from homozygous mice. (J:123680, J:262494)