Gt(ROSA)26Sor^{tm1.1(Cyp1a1,Thy1)Stck}

A minigene composed of the coding sequences of mouse Cyp1a1 and rat Thy1 connected by a 2A sequence followed by the Woodchuck hepatitis virus derived regulatory element and a bovine growth hormone polyadenylation site was inserted into the locus. In addition a cassette containing an FRT-flanked neomycin-resistance gene and loxP-flanked transcriptional stop elements were inserted upstream of the minigene. Flp-mediated recombination removed the neomycin resistance gene, leaving the floxed STOP cassette. This creates a conditional-ready allele that will only be expressed after cre-mediated deletion of the STOP cassette. (J:244974)