Tg(hs643-cre/ERT2,-GFP)838Jlr

This transgene contains the human enhancer element hs643 -- which is flanked on human Chr 9 by the long intergenic non-protein coding RNA 1239 (LINC01239) and ELAV like RNA binding protein 2 (ELAVL2) loci -- followed by a mouse minimal Hsp68 promoter, the cre/ERT2 fusion gene, an internal ribosomal entry site (IRES), and a green fluorescent protein reporter gene. The fusion gene encodes Cre recombinase joined to a modified ligand-binding domain of the human estrogen receptor that preferentially binds tamoxifen or 4-hydroxytamoxifen, but not endogenous 17beta-estradiol; upon binding to tamoxifen, the cre/ERT2 protein is translocated to the nucleus. (J:214335)