CRISPR/cas9基因编辑通过引导RNA定向靶向Gt(ROSA)26Sor基因座的内含子1。构建了由非同源的attP锚定位点(如48bp的Bxb1 attP-GT序列、240bp的Gt(ROSA)26Sor序列的天然间隔区和48bp的Bxb1 attP-GA序列)环绕的寡核苷酸供体DNA。来源:(J:322700)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ
Endonuclease-mediated
插入
不确定
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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