The targeting cassette contains CAG-loxP-(PGK-neomycin - 4x polyA) Stop-loxP-biotin ligase recognition peptide (BLRP)-3x FLAG-LSD1 WT-IRES2-EGFP-4x polyA. The LSD1 VN (KDM1A) insertion expresses a FLAG-tagged full-length human KDM1A cDNA, which encodes an 852 amino acid isoform of the gene that includes a 60 nucleotide exon in intron 2 (E2a) as well as an EGFP fluorescent reporter. (J:101977)