通过GENSAT修饰的细菌人工染色体(BAC)RP23-224O8,其编码增强绿色荧光蛋白(EGFP)的序列被插入到了小鼠神经前体细胞盒2(Nkx2.2)基因5'调控序列的下游,来自CHORI,然后用于对受精卵的精子核进行微注射。后代中, founder line 2 显示出了最强的EGFP表达。(来源:J:214964)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count