Tg(Igk-mNeonGreen/mRuby2)50.28Mnz

The reporter cDNA is introduced to mouse Igk regulatory elements: Vkappa21E promoter-noncoding leader (L) exon-Vkappa exon-mNeonGreen/mRuby2 (INDIA) cDNA-polyA-Jkappa5 intronic sequence with enhancer (ie) and 3' regulatory region (rr). The reporter consists of an optimized fluorescence (Forster) resonance energy transfer (FRET) pair: N-terminally Myc-tagged mNeonGreen and mRuby2, linked by an 18 amino acid peptide containing an aCasp3-cleavage site (DEVDG). When aCasp3 (Casp3, caspase 3; a marker for apoptosis) cleaves the linker, mNeonGreen and mRuby2 are separated, resulting in FRET loss and increased emission from mNeonGreen. Non-apoptotic cells retaining uncleaved mNeonGreen/mRuby2 demonstrate more yellow fluorescence indicating a lower ratio of mNeonGreen/FRET fluorescence emission. (J:261534)