这个来自TCPR0848项目的等位基因是在表观基因组学中心通过电穿孔,使用了带有ACACGGGCGAGGAGTAAGAG和TTATGCCATTTGTGATGAGC的单导RNA,针对7号染色体107,714,456到107,714,777的位置,目标是5'端,以及CACAGCGACCATGCAGAGCG和GAGTATGTCCTTGCATGTCT,针对3'端的某个关键区域。这导致了7号染色体107,714,456到107,714,777一段322bp的缺失(GRCm38建库)。(引用:J:237616)
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This allele from project TCPR0848 was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of ACACGGGCGAGGAGTAAGAG and TTATGCCATTTGTGATGAGC targeting the 5' side and CACAGCGACCATGCAGAGCG and GAGTATGTCCTTGCATGTCT targeting the 3' side of a critical region. This resulted in a 322-bp deletion from Chr7:107714456 to 107714777 (GRCm38). (J:237616)