Stat2^{tm1.1(STAT2)Diam}

The targeting vector was created using mouse Stat2 sequences from C57BL/6J BAC RPCIB-731 and human STAT2 sequences from human BAC RPCIB-753. The targeting vector also inserted an FRT-flanked neomycin resistance cassette in intron 1 and a F3-flanked puromycin resistance cassette in intron 24. The vector is designed replace the endogenous mouse Stat2 coding sequence (spanning from part of exon 2 through part of exon 24) with the corresponding human STAT2 coding sequence. The allele retains the endogenous mouse Stat2 5' untranslated region, promoter sequences (exon 1 and part of exon 2) and 3' untranslated region. Flp-mediated recombination removed the FRT-flanked neo and puro cassettes. (J:261503)