这个来自TCPR0783项目的研究等位基因是在表型基因组学中心通过电穿孔,使用了带有CGGCCCTCCCTTGTCTAAAA和CGTCACCAGAGGCATAAATG指导RNA的Cas9核糖核酸蛋白复合物,目标是5'端和3'端的特定区域。这导致了7号染色体49,408,477到49,408,949位置的473bp缺失,以及49,409,000到49,409,270位置的271bp缺失(GRCm38参考序列,J:237616)。
查看原文 参与反馈
This allele from project TCPR0783 was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of CGGCCCTCCCTTGTCTAAAA and CGTCACCAGAGGCATAAATG targeting the 5' side and GGCGCTGCCGACCCATCTTC and CCAATCAGACCAGTGGCGCT targeting the 3' side of a critical region. This resulted in a 473-bp deletion of Chr7 from 49408477 to 49408949 and a 271-bp deletion of Chr7 from 49409000 to 49409270 (GRCm38). (J:237616)