这个来自TCPR0859项目的等位基因是在表型遗传学中心通过电穿孔,使用了带有AGGACGGCTTCGTAGGCCAG和CCGGAAAACCTGCAGACATG的单链RNA指导蛋白复合物,目标是1'端,以及CTAGGAACGAATCTCAGAAC和GTTACTTACCCATGAGGCTT的单链RNA,针对3'端。这导致了11号染色体1,518,354,90到1,518,357,88位置的299bp缺失,同时在11号染色体的1,518,353,95位置有一个delCinsAGTAAGTA的变异(GRCm38建库,J:237616)。
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This allele from project TCPR0859 was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of AGGACGGCTTCGTAGGCCAG and CCGGAAAACCTGCAGACATG targeting the 5' side and CTAGGAACGAATCTCAGAAC and GTTACTTACCCATGAGGCTT targeting the 3' side. This resulted in a 299-bp deletion Chr11:51835490 to 51835788 and Chr11:51835395_delCinsAGTAAGTA (GRCm38). (J:237616)