这个来自TCPR0902项目的研究等位基因是在表型遗传学中心通过电穿孔,使用了带有GACCACAGTCAAGGTCCAAC和AATAGGGCTGGATGTAATCC的单导RNA,针对染色体15的5'端,以及GGCCACAGTACACTACTGAT和AGGTGATCCAATAACCTAAA针对3'端的特定外显子(或可能多个)进行的。这导致了15号染色体3,159,5629到3,159,6023位置的395bp缺失,以及15号染色体3,159,5496位置的一个插入型变异(GRCm38建库,J:237616)。
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This allele from project TCPR0902 was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of GACCACAGTCAAGGTCCAAC and AATAGGGCTGGATGTAATCC targeting the 5' side and GGCCACAGTACACTACTGAT and AGGTGATCCAATAACCTAAA targeting the 3' side of a critical exon(s). This resulted in a 395-bp deletion of Chr15 from 31595629 to 31596023 and and indel at Chr15:31595496_insTTG (GRCm38). (J:237616)