将包含一段10,389bp的 mouse Gad2 基因exon 16的基因组DNA片段,以及插入在终止密码子TAA和3' UTR之间的IRES-FlpO-loxP2272-ACE-Cre POII NeoR-loxp2272 cassette的靶向载体,导入ES细胞中。阳性ES克隆经扩增后,用于产生经过NeoR cassette自噬的嵌合小鼠。(来源:J:261454)
Basic Information
(129S6/SvEvTac x C57BL/6J)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count