该向量从5'至3'设计,包含FRT3位点、两个鸡β-球蛋白HS4绝缘子元件(用于在无转录激活剂时降低报告基因表达)、tet响应元件/启动子、一个含三读框终止子的floxed-STOP序列(与合成的pA-hGHpA-PGKpA相连的终止密码子),ChR2(H134R)-EYFP融合序列、WPRE(增强mRNA稳定性)、BGHpolyA、两个鸡β-球蛋白HS4绝缘子元件、AttB位点、一个PGK-5'hygro插入片段、RNA剪接供体以及FRT5位点。通过ΦC31介导的重组,移除了AttB/AttP-介导的序列,并替换为重组的AttB/AttP(AttL)位点。来源:J:260362

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(129S6/SvEvTac x C57BL/6NCrl)F1
Targeted
插入
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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